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Suzhou Vocational Health College

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Quantifying the Level of 8-oxo-dG Using ELISA Assay to Evaluate Oxidative DNA Damage in MCF-7 Cells
Liu Nian *1,2, Xiaohua Li *3, Jiahui Du 1, Song-Bai Liu 1,2
1Suzhou Key Laboratory of Medical Biotechnology, Suzhou Vocational Health College, 2Department of Chemistry and Life Sciences, Suzhou University of Science and Technology, 3Department of Thyroid and Breast Surgery, Wuzhong People’s Hospital of Suzhou City

This protocol describes an efficient method for quantitatively detecting DNA oxidative damage in MCF-7 cells by ELISA technology.

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