University of Antioquia

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

Sara M. Robledo¹, Lina M. Carrillo^1,2, Alejandro Daza¹, Adriana M. Restrepo¹, Diana L. Muñoz¹, Jairo Tobón¹, Javier D. Murillo¹, Anderson López¹, Carolina Ríos¹, Carol V. Mesa¹, Yulieth A. Upegui¹, Alejandro Valencia-Tobón¹, Karina Mondragón-Shem¹, Berardo RodrÍguez², Iván D. Vélez¹

¹Program for the Study and Control of Tropical Diseases -PECET-School of Medicine, University of Antioquia, ²School of Agrarian Sciences, University of Antioquia

Optimization of the experimental hamster model for cutaneous leishmaniasis by intradermal injection of Leishmania promastigotes at the dorsal skin. This approach is useful during inoculation, follow-up, characterization of lesions, application of treatments and obtaining of clinical samples. Locomotion, search for food and water, play and social activities are preserved.

Bioengineering

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles

Sandra L. Arias¹, Akshath R. Shetty², Angana Senpan³, Mónica Echeverry-Rendón⁴, Lisa M. Reece^5,6, Jean Paul Allain^1,2,3,7

¹Department of Bioengineering, University of Illinois at Urbana-Champaign, ²Department of Nuclear, Plasma and Radiological Engineering, University of Illinois at Urbana-Champaign, ³Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, ⁴Program of Study and Control of Tropical Diseases (PECET), University of Antioquia, ⁵Sealy Center for Vaccine Development, University of Texas Medical Branch, ⁶WHO Collaborating Center for Vaccine Research, Evaluation and Training on Emerging Infectious Diseases, University of Texas Medical Branch, ⁷Beckman Institute, University of Illinois at Urbana-Champaign

Here, we present a protocol to make a bacterial nanocellulose (BNC) magnetic for applications in damaged blood vessel reconstruction. The BNC was synthesized by G. xylinus strain. On the other hand, magnetization of the BNC was realized through in situ precipitation of Fe²⁺ and Fe³⁺ ferrous ions inside the BNC mesh.

Biology

Intact Short, Intermediate, and Long Skeletal Muscle Fibers Obtained by Enzymatic Dissociation of Six Hindlimb Muscles of Mice: Beyond Flexor Digitorum Brevis

Jorge L. Petro^*1, Andrés F. Milán^*1, Erika Arenas¹, Laura Valle¹, Valeria Hernández¹, Juan C. Calderón¹

¹Physiology and Biochemistry Research Group-PHYSIS, Faculty of Medicine, University of Antioquia

We describe a protocol to obtain enzymatically dissociated fibers of different lengths and types from six muscles of adult mice: three of them already described (flexor digitorum brevis, extensor digitorum longus, soleus) and three of them successfully dissociated for the first time (extensor hallucis longus, peroneus longus, peroneus digiti quarti).