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Max Planck Institute for Heart and Lung Research

2 ARTICLES PUBLISHED IN JoVE

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Biology

Miniaturized Sample Preparation for Transmission Electron Microscopy
Claudio Schmidli *1,2, Luca Rima *1, Stefan A. Arnold *1,2, Thomas Stohler 1, Anastasia Syntychaki 1, Andrej Bieri 1, Stefan Albiez 1, Kenneth N. Goldie 1, Mohamed Chami 3, Henning Stahlberg 1, Thomas Braun 1
1Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, 2Swiss Nanoscience Institute, University of Basel, 3BioEM lab, Biozentrum, University of Basel

An instrument and methods for the preparation of nanoliter-sized sample volumes for transmission electron microscopy is presented. No paper-blotting steps are required, thus avoiding the detrimental consequences this can have for proteins, significantly reducing sample loss and enabling the analysis of single cell lysate for visual proteomics.

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Biochemistry

Identification of Functional Protein Regions Through Chimeric Protein Construction
Juan M. Adrian-Segarra 1, Holger Lörchner 1,2, Thomas Braun 1,2, Jochen Pöling 1,2
1Department of Cardiac Development and Remodelling, Max Planck Institute for Heart and Lung Research, 2Partner site Rhein-Main, German Centre for Cardiovascular Research (DZHK)

Structurally related proteins frequently exert distinct biological functions. The exchange of equivalent regions of these proteins in order to create chimeric proteins constitutes an innovative approach to identify critical protein regions that are responsible for their functional divergence.

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