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South Dakota State University

4 ARTICLES PUBLISHED IN JoVE

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Environment

How to Create Conditioned Taste Aversion for Grazing Ground Covers in Woody Crops with Small Ruminants
Carmen L. Manuelian 1, Elena Albanell 1, Maristela Rovai 1,2, Gerardo Caja 1
1Group of Ruminant Research (G2R), Department of Animal and Food Science, Universitat Autònoma de Barcelona, 2Dairy Science Department, South Dakota State University

Feeding behavior preferences in livestock can be modified to implement a grazing management plan in woody crops. Here, we present a protocol to show a lithium chloride dose after eating a new plant that induces conditioned taste aversion.

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Education

Protocol for Assessing the Relative Effects of Environment and Genetics on Antler and Body Growth for a Long-lived Cervid
Eric S. Michel 1,2, Emily B. Flinn 1, Stephen Demarais 1, Bronson K. Strickland 1, Guiming Wang 1, Chad M. Dacus 3
1Department of Wildlife, Fisheries and Aquaculture, Mississippi State University, 2Department of Natural Resource Management, South Dakota State University, 3Mississippi Department of Wildlife, Fisheries and Parks

Phenotypic differences among cervid populations may be related to population-level genetics or nutrition; discerning which is difficult in the wild. This protocol describes how we designed a controlled study where nutritional variation was eliminated. We found that phenotypic variation of male white-tailed deer was more limited by nutrition than genetics.

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Environment

In Situ Soil Moisture Sensors in Undisturbed Soils
Todd G. Caldwell 1, Michael H. Cosh 2, Steven R. Evett 3, Nathan Edwards 4, Heather Hofman 5, Bradley G. Illston 6, Tilden Meyers 7, Marina Skumanich 8, Kent Sutcliffe 9
1Nevada Water Science Center, U.S. Geological Survey, 2Agricultural Research Service, Hydrology and Remote Sensing Lab., U.S. Department of Agriculture, 3Agricultural Research Service, Conservation and Production Research Lab., U.S. Department of Agriculture, 4South Dakota State University, 5National Water and Climate Center, U.S. Department of Agriculture, 6University of Oklahoma, 7Air Resources Lab, National Oceanic and Atmospheric Administration, 8National Integrated Drought Information System, National Oceanic and Atmospheric Administration, 9Snow Survey Staff, U.S. Department of Agriculture

The determination of soil water content is a critical mission requirement for many state and federal agencies. This protocol synthesizes multi-agency efforts to measure soil water content using buried in situ sensors.

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Cancer Research

Growing Desmoplastic Three-Dimensional Pancreatic Cancer Spheroids from Co-Culture
Rishabh Bahl 1, Cindy Venegas Mata 1, Benedikt Neth 1, Lara Meinzinger 1, Annika Celine Deppe 1, Haley Jahnke 1, Camryn Blackwell 1, Mikhail Durymanov 1, Joshua Reineke 1
1Department of Pharmaceutical Sciences, South Dakota State University

Pancreatic cancer remains one of the toughest cancers to treat. Therefore, it is critical that pre-clinical models evaluating treatment efficacy are reproducible and clinically relevant. This protocol describes a simple co-culture procedure to generate reproducible, clinically relevant desmoplastic spheroids.

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