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Immunology and Infection

Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
F. Liaini Gross 1,2, Yaohui Bai 1, Stacie Jefferson 1, Crystal Holiday 1, Min Z. Levine 1
1Influenza Division, Centers for Disease Control and Prevention, 2Battelle

Influenza neutralizing antibodies correlate with protection of influenza infections. Microneutralization assays measure neutralizing antibodies in human sera and are often used for influenza human serology. We describe a microneutralization assay using MDCK-SIAT1 cells to measure neutralizing antibody titers to contemporary 3C.2a and 3C.3a A(H3N2) viruses following influenza vaccination or infection.

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