Toyo University

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs

Chisato Kataoka^1,2, Shosaku Kashiwada^1,2

¹Graduate School of Life Sciences, Toyo University, ²Research Center for Life and Environmental Sciences, Toyo University

Embryonic stages are the most susceptible to xenobiotics. Although chemical toxicity depends on salinity, no method exists to test the salinity dependence of toxicity to aquatic organisms. Here, we describe a new and high-throughput method for determining the salinity dependence of toxicity to aquatic embryos.

Environment

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac

Imari Koike¹, Koichiro Shimomura¹, Mikihisa Umehara¹

¹Graduate School of Life Sciences, Toyo University

Adventitious shoots can be induced on internodal segments of ipecac without phytohormone treatment. To evaluate phytohormone dynamics during adventitious shoot formation, we measured endogenous auxin and cytokinin in internodal segments by LC-MS/MS.