In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane DyeMeng Li *1,2, Ming Hao *1,2, Dong Jiang 3, Yanxi Chen 4, Wen Wang 1,2
1Cellular Biomedicine Group, China, 2Cellular Biomedicine Group, California, 3Department of Orthopedics, Shanghai TCM-Integrated Hospital, 4Department of Orthopedics, Shanghai East Hospital, Tongji University
This protocol describes an efficient way to monitor the cell persistence and biodistribution of human adipose-derived mesenchymal stem cells (haMSCs) by far-red fluorescence labeling in a rat knee osteoarthritis (KOA) model via intra-articular (IA) injection.