Bernhard Nocht Institute for Tropical Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Forced Salivation As a Method to Analyze Vector Competence of Mosquitoes

Anna Heitmann^*1, Stephanie Jansen^*1,2, Renke Lühken¹, Mayke Leggewie^1,2, Jonas Schmidt-Chanasit^1,2, Egbert Tannich^1,2

¹Bernhard Nocht Institute for Tropical Medicine, ²German Centre for Infection Research (DZIF)

For efficient control of mosquito borne virus transmission, the knowledge of the vector potential of respective mosquitoes is of particular interest. We describe forced salivation as a method to analyze vector competence of Aedes albopictus and three different Culex taxa for the transmission of Zika virus.

Immunology and Infection

Experimental Infection of Mice with the Parasitic Nematode Strongyloides ratti

Sara Dörken¹, Kira Duhm¹, Birte Viebrock¹, Lara Linnemann¹, Lennart Heepmann¹, Wiebke Hartmann¹, Jennifer Antwi-Ekwuruke¹, M. Saleh Yunus^1,2, Minka Breloer^1,3

¹Section Interface, Helminth Immunology Group, Bernhard Nocht Institute for Tropical Medicine, ²Institute for Infection Research and Vaccine Development, University Hospital Eppendorf, ³Department for Biology, University Hamburg

Strongyloides ratti is a parasitic nematode that causes transient infections in laboratory mice, displaying tissue-migrating and intestinal life stages. Here, we present a protocol for the maintenance of the parasite cycle in rats and experimental infection of mice, including parasite quantification in the head, lung, and intestine.