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Tsurumi University School of Dental Medicine

2 ARTICLES PUBLISHED IN JoVE

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Genetics

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
Takatoshi Murata 1, Ayako Okada 1, Khairul Matin 1,2,3, Nobuhiro Hanada 1
1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

We describe a facile, rapid, and relatively inexpensive method for the generation of gene-disrupted Streptococcus mutans strains; this technique may be adapted for the generation of gene-disrupted strains of various species.

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Immunology and Infection

Purification of a High Molecular Mass Protein in Streptococcus mutans
Takatoshi Murata 1, Mamiko Yamashita 1, Masao Ishikawa 1,2, Koji Shibuya 2, Nobuhiro Hanada 1
1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Laboratory for Oral Health Science

Described here is a simple method for the purification of a gene product in Streptococcus mutans. This technique may be advantageous in the purification of proteins, especially membrane proteins and high molecular mass proteins, and can be used with various other bacterial species.

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