Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host SpeciesQiongxia Lyu 1,2, Huifei Sophia Zheng 2, Karly Laprocina 2, Chen-Che Jeff Huang 2
1College of Animal Science & Technology, Henan University of Science and Technology, 2Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University
Several different methods have been established for multiplex immunostaining using primary antibodies from the same host species. Here, we describe the use of microwave-mediated antibody stripping and of fluorophore-tyramide to block antibody cross-reactivity during multiplex immunostaining on formalin-fixed paraffin-embedded mouse adrenal sections.
