Xuanwu Hospital Capital Medical University

11 ARTICLES PUBLISHED IN JoVE

Neuroscience

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

Ying Wang¹, Nicholas Baker¹, Gro V. Amdam^1,2

¹School of Life Sciences, Arizona State University , ²Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences

In this protocol, we describe two strategies that simultaneously suppress two genes (double gene knockdown) in honey bees. Then we present how to use the proboscis extension response (PER) assay to study the effect of double gene knockdown on honey bee gustatory perception.

Immunology and Infection

In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging

Yucheng Wang^1,2,3, Olivia D. Harrington¹, Ying Wang¹, Clinton K. Murray⁴, Michael R. Hamblin¹, Tianhong Dai¹

¹Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, ²Department of Medical Oncology, Beijing Institute of Translational Medicine, Chinese Academy of Sciences, ³Cancer Center, Aviation General Hospital, Beijing, ⁴Infectious Disease Service, Brooke Army Medical Center

Infections caused by multidrug-resistant (MDR) bacterial strains have emerged as a serious threat to public health, necessitating the development of alternative therapeutics. We present a protocol to evaluate the effectiveness of antimicrobial blue light (aBL) therapy for MDR Acinetobacter baumannii infections in mouse burns by using bioluminescence imaging.

Cancer Research

A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay

Ying Wang¹, DanQi Chen¹, MingYu Chen¹, KaiYuan Ji¹, DeXuan Ma¹, LiangFu Zhou¹

¹Department of Neurosurgery, Huashan Hospital, Fudan University

This paper presents a comprehensive procedure to evaluate in vitro whether classic tumor angiogenesis exists in hemangioblastomas (HBs) and its role in HBs. The results highlight the complexity of HB-neovascularization and suggest that this common form of angiogenesis is only a complementary mechanism in the HB-neovascularization.

Engineering

Radio Frequency Magnetron Sputtering of GdBa₂Cu₃O₇₋_δ/ La_0.67Sr_0.33MnO₃ Quasi-bilayer Films on SrTiO₃ (STO) Single-crystal Substrates

Ying Wang^1,2, Zhen Li^1,2, YongSheng Liu¹, Yijie Li², Linfei Liu², Da Xu², Xiaojing Luo¹, Tian Gao^1,3, Yanyan Zhu^1,4, Luozeng Zhou³, Jianming Xu³

¹Department of Physics, Mathematics, Shanghai Key Laboratory of Materials Protection and Advanced Materials in Electric Power, Shanghai University of Electric Power, ²Key Laboratory of Artificial Structure and Quantum Control, Ministry of Education, Department of Physics, Shanghai Jiao Tong University, ³Shanghai Institute of Space Power-sources, ⁴Shanghai Key Laboratory of High Temperature Superconductors, Shanghai University

Here, we present a protocol to grow LSMO nanoparticles and (Gd) BCO films on (001) SrTiO₃ (STO) single-crystal substrates by radio frequency (RF)-sputtering.

Immunology and Infection

Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay

Caiyun Liu¹, Mingqin Zhu¹, Ying Wang¹

¹Department of Neurology and Neuroscience Center, The First Hospital of Jilin University

Cell-based assay is a widely used method to detect serum anti-aquaporin-4 immunoglobulin G. This method could be applied to clinical diagnosis and scientific researches of neuromyelitis optical spectrum disorders.

Genetics

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Ying Wang^*1,2, Zheng Kuang^*1,2, Lei Li², Xiaozeng Yang¹

¹Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, ²State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University

A bioinformatics pipeline, namely miRDeep-P2 (miRDP2 for short), with updated plant miRNA criteria and an overhauled algorithm, could accurately and efficiently analyze microRNA transcriptomes in plants, especially for species with complex and large genomes.

Immunology and Infection

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

Soichi Sano¹, Ying Wang¹, Megan A. Evans¹, Yoshimitsu Yura¹, Miho Sano¹, Hayato Ogawa¹, Keita Horitani¹, Heather Doviak¹, Kenneth Walsh¹

¹Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine

Described are protocols for the highly efficient genome editing of murine hematopoietic stem and progenitor cells (HSPC) by the CRISPR/Cas9 system to rapidly develop mouse model systems with hematopoietic system-specific gene modifications.

JoVE Core

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

Eunbee Park¹, Megan A. Evans⁴, Heather Doviak⁴, Keita Horitani⁴, Hayato Ogawa⁴, Yoshimitsu Yura⁴, Ying Wang², Soichi Sano^3,4, Kenneth Walsh^1,4

¹Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, ²Department of Cardiology, Xinqiao Hospital, Army Medical University, ³Department of Cardiology, Osaka City University Graduate School of Medicine, ⁴Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine

We describe three methods of bone marrow transplantation (BMT): BMT with total-body irradiation, BMT with shielded irradiation, and BMT method with no pre-conditioning (adoptive BMT) for the study of clonal hematopoiesis in mouse models.

Bioengineering

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology

Sheng-Chang Luo^1,2, Ying Wang³, Ranjith Kumar Kankala^1,2, Yu Shrike Zhang⁴, Ai-Zheng Chen^1,2

¹Institute of Biomaterials and Tissue Engineering, Huaqiao University, ²Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, ³Affiliated Dongguan Hospital, Southern Medical University, ⁴Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School

The present protocol describes the fabrication of poly(lactic-co-glycolic acid)-based highly open porous microspheres (HOPMs) via the single-emulsion formulation based facile microfluidic technology. These microspheres have potential applications in tissue engineering and drug screening.

Neuroscience

Using a Bipolar Electrode to Create a Temporal Lobe Epilepsy Mouse Model by Electrical Kindling of the Amygdala

Yongchang Lu^*1,2, Yang Dai^*1,2, Siqi Ou^1,2, Yujing Miao¹, Ying Wang^1,2, Quanlei Liu^1,2, Yihe Wang^1,2, Penghu Wei^1,2, Yongzhi Shan^1,2, Guoguang Zhao^1,2,3

¹Department of Neurosurgery, Xuanwu Hospital Capital Medical University, ²China Clinical Research Center for Epilepsy Capital Medical University, ³China Beijing Municipal Geriatric Medical Research Center

The amygdala plays a key role in temporal lobe epilepsy, which originates in and propagates from this structure. This article provides a detailed description of the fabrication of deep brain electrodes with both recording and stimulating functions. It introduces a model of medial temporal lobe epilepsy originating from the amygdala.

Medicine

In Vitro Application of a Wireless Sensor in Flexion-Extension Gap Balance of Unicompartmental Knee Arthroplasty

Xufeng Jiao¹, Yunfei Jiang¹, Zheng Li¹, Shuai An¹, Jiang Huang¹, Guanglei Cao¹

¹Department of Orthopedics, Xuanwu Hospital Capital Medical University

This protocol presents a cadaveric study of a wireless sensor used in medial unicompartmental knee arthroplasty. The protocol includes the installation of an angle measuring device, standardized Oxford unicompartmental knee arthroplasty osteotomy, preliminary assessment of flexion-extension balance, and application of the sensor to measure flexion-extension gap pressure.