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Zhengzhou University

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
Ming Yuan *1, Pengju Wang *2,3, Louisa S. Chard 1, Nicholas R. Lemoine 1,2,3, Yaohe Wang 1,2,3
1Center for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, 2Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy, Zhengzhou University, 3School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University

Vaccinia virus (VV) has been widely used in biomedical research and the improvement of human health. This article describes a simple, highly efficient method to edit the VV genome using a CRISPR-Cas9 system.

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Bioengineering

Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
Rong Li *1, Jinxin Miao *1,2, Zhiqiang Fan 1, SeokHwan Song 3, Il-keun Kong 3,4, Yaohe Wang 2,5, Zhongde Wang 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London

Pronuclear (PN) injection of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system is a highly efficient method for producing genetically engineered golden Syrian hamsters. Herein, we describe the detailed PN injection protocol for the production of gene knockout hamsters with the CRISPR/Cas9 system.

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Bioengineering

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
Mingjun Xie 1,2, Kang Yu 1,2, Yuan Sun 1,2, Lei Shao 1,2, Jing Nie 1,2, Qing Gao 1,2, Jingjiang Qiu 3, Jianzhong Fu 1,2, Zichen Chen 1,2, Yong He 1,2
1State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, 2Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, 3School of Mechanics and Safety Engineering, Zhengzhou University

Presented here is a method for the 3D bioprinting of gelatin methacryloyl.

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Immunology and Infection

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
Hongzheng Bi 1,2, Samiksha Wasnik 1, David J. Baylink 1, Chenfan Liu 1,3,4, Xiaolei Tang 1,3
1Division of Regenerative Medicine, Department of Medicine, Loma Linda University, 2Zhengzhou University, 3Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, 4Jinan Infectious Disease Hospital, Shandong University

Here we present a protocol for in vivo augmentation of gut-homing regulatory T cell induction. In this protocol, dendritic cells are engineered to locally produce high concentrations of the active vitamin D (1,25-dihydroxyvitamin D or 1,25[OH]2D) and the active vitamin A (retinoic acid or RA) de novo.

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Immunology and Infection

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
Liang Wang *1,2,3, Jin-Xin Lai *1, Yu-Ting Si *1,4, Xu-Xia Cui 1,4, Zeeshan Umar 1,5, Xiao-Jun Ru 1, Xin-Yu Zhang 1, Zheng-Kang Li 1, Alfred Chin Yen Tay 5,6,7,8, Barry J. Marshall 5,6,7,8, Guang-Hua Li 1, Bing Gu 1
1Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, 2Division of Microbiology and Immunology, School of Biomedical Sciences, The University of Western Australia, 3Center for Precision Health, School of Medical and Health Sciences, Edith Cowan University, 4Medical Technology School of Xuzhou Medical University, 5Marshall Laboratory of Biomedical Engineering, School of Medicine, Shenzhen University, 6The Marshall Centre for Infectious Diseases Research and Training, The University of Western Australia, 7Marshall International Digestive Diseases Hospital, Zhengzhou University, 8Marshall Medical Research Center, Fifth Affiliated Hospital of Zhengzhou University

The protocol presents a noninvasive method for the rapid diagnosis of Helicobacter pylori stomach infections through the string test and determines its antibiotic resistance to clarithromycin and levofloxacin using quantitative polymerase chain reaction (qPCR).

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