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IMIB-Arrixaca

4 ARTICLES PUBLISHED IN JoVE

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Medicine

Ultrasonography in Experimental Reproductive Investigations on Rats
Tianjuang Wang 1,2, Lidia Oltra-Rodríguez 3, Nuria García-Carrillo 3, Anibal Nieto 4,5, Yunxia Cao *1,2,6, María L. Sánchez-Ferrer *4,5
1Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, 2Institute of Reproductive Genetics, Anhui Medical University, 3Centro Experimental en Investigaciones Biomédica (CEIB), Campus Ciencias de la Salud, Universidad de Murcia, 4Department of Obstetrics and Gynecology, 'Virgen de la Arrixaca' University Clinical Hospital, 5Institute for Biomedical Research of Murcia, IMIB-Arrixaca, 6Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs

This manuscript describes the utility of ultrasound performed on female rats to design experimental models for reproductive and gynecological investigation. A step-by-step explanation of how to perform ultrasonographic evaluation is shown.

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Medicine

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing
Sergio Liarte *1, Ángel Bernabé-García *1, David Armero-Barranco 2, Francisco José Nicolás 1
1Laboratorio de Oncología Molecular y TGF-β, IMIB-Arrixaca, 2Departamento de Enfermería, Facultad Enfermería, Universidad de Murcia

This manuscript describes how incision-like lesions made on cultured epithelial cell monolayers conveniently model wound healing in vitro, allowing for imaging by confocal or laser scanning microscopy, and which can provide high-quality quantitative and qualitative data for studying both cell behavior and the mechanisms involved in migration.

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Medicine

Anogenital Distance and Perineal Measurements of the Pelvic Organ Prolapse (POP) Quantification System
María Luisa Sánchez-Ferrer 1,2, María Teresa Prieto-Sánchez 1,2, Carlos Moya-Jiménez 1, Jaime Mendiola 2,3,4, Carmen María García-Hernández 1, Ana Carmona-Barnosi 1, Anibal Nieto 1,2, Alberto M. Torres-Cantero 2,3,4,5
1Department of Obstetrics & Gynecology, "Virgen de la Arrixaca", University Clinical Hospital, 2Institute for Biomedical Research of Murcia, IMIB-Arrixaca, 3Division of Preventive Medicine and Public Health, Department of Public Health Sciences, University of Murcia School of Medicine, 4Biomedical Research Centre Network for Epidemiology and Public Health (CIBERESP), 5Department of Preventive Medicine, "Virgen de la Arrixaca", University Clinical Hospital

This manuscript describes the procedures to perform anogenital distance (AGD) and perineal measurements standardized by the Pelvic Organ Prolapse Quantification System (POP-Q): perineal body (PB) and genital hiatus (GH). These measurements are compared in women with and without pelvic organ prolapse.

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Medicine

External Cephalic Version: Is it an Effective and Safe Procedure?
Javier Sánchez-Romero 1, Vanesa García-Soria 2, Fernando Araico-Rodríguez 1, Javier Herrera-Giménez 1, José E. Blanco-Carnero 1,3, Aníbal Nieto-Díaz 1,3,4, María Luisa Sánchez-Ferrer 1,3,4
1Department of Obstetrics & Gynecology, "Virgen de la Arrixaca", University Clinical Hospital, 2Department of Gynecology, "Reina Sofía", University Clinical Hospital, 3Department of Obstetrics and Gynecology, Pediatrics and Surgery, University of Murcia, 4Institute for Biomedical Research of Murcia

This article shows how to perform the external cephalic version (ECV) by two experienced obstetricians in the obstetric operating room in the presence of an anesthesiologist and a midwife. The ECV is carried out with analgesia and tocolysis. Two attempts are made under ultrasonography control.

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