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Jagiellonian University

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
Anna Kula-Pacurar 1, Jakub Wadas 2, Agnieszka Suder 1, Artur Szczepanski 1,2, Aleksandra Milewska 1,2, Marek Ochman 3,4, Tomasz Stacel 3,4, Krzysztof Pyrc 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

Here, we describe a simple method that combines RNA fluorescence in situ hybridization (RNA-FISH) with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. This protocol may increase understanding of the molecular characteristics of SARS-CoV-2 RNA-host interactions at a single-cell level.

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Chemistry

Microscopic Visualization of Porous Nanographenes Synthesized through a Combination of Solution and On-Surface Chemistry
Rafal Zuzak 1, Iago Pozo 2, Mads Engelund 3, Manuel Vilas-Varela 2, José M. Alonso 2, Enrique Guitián 2, Dolores Pérez 2, Diego Peña 2, Szymon Godlewski 1
1Centre for Nanometer-Scale Science and Advanced Materials, NANOSAM, Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 2Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, 3Espeem S.A.R.L.

A combination of solution and surface assisted synthesis opens new directions in the atomically precise synthesis of nanostructures. Scanning tunneling microscopy (STM) supplemented by non-contact atomic force microscopy (nc-AFM) enables detailed characterization of newly designed and generated carbon-based nano-objects.

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Medicine

Application of Lucilia sericata Larvae in Debridement of Pressure Wounds in Outpatient Settings
Dariusz Bazaliński 1,2, Paweł Więch 2, Paulina Szymańska 3, Marek Muster 2, Maria Kózka 4
1Father B. Markiewicz Podkarpackie Specialist Oncology Centre, Specialist Hospital in Brzozów, 2Institute of Health Sciences, College of Medical Sciences, University of Rzeszów, 3General Surgery Clinic, Specialist Hospital in Radom, 4Department of Clinical Nursing, Faculty of Health Sciences, Collegium Medicum, Jagiellonian University

Maggots of Lucilia sericata were used in the debridement of deep pressure sores in outpatient settings for the treatment of pressure sores involving the complete skin thickness. The technique presented in the article does not pose risks for the patient.

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Immunology and Infection

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
Aneta Babula *1,2, Izabela Siemińska *1,3, Monika Baj-Krzyworzeka 1
1Department of Clinical Immunology, Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences, Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU, University of Agriculture in Kraków

The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them.

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Neuroscience

A Naturalistic Setup for Presenting Real People and Live Actions in Experimental Psychology and Cognitive Neuroscience Studies
Tuğçe Nur Pekçetin 1, Şeyda Evsen 2, Serkan Pekçetin 3, Cengiz Acarturk 4, Burcu A. Urgen 5
1Department of Cognitive Science, Middle East Technical University, 2Interdisciplinary Neuroscience Graduate Program, Bilkent University, 3Department of Computer Engineering, Middle East Technical University, 4Department of Cognitive Science, Jagiellonian University, 5Department of Psychology, Interdisciplinary Neuroscience Graduate Program, National Magnetic Resonance Research Center (UMRAM), Aysel Sabuncu Brain Research Center, Bilkent University

This study presents a naturalistic experimental setup that allows researchers to present real-time action stimuli, obtain response time and mouse tracking data while participants respond after each stimulus display, and change actors between experimental conditions with a unique system including a special transparent organic light-emitting diode (OLED) screen and light manipulation.

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