Jagiellonian University

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Anna Kula-Pacurar¹, Jakub Wadas², Agnieszka Suder¹, Artur Szczepanski^1,2, Aleksandra Milewska^1,2, Marek Ochman^3,4, Tomasz Stacel^3,4, Krzysztof Pyrc¹

¹Malopolska Centre of Biotechnology, Jagiellonian University, ²Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ³Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, ⁴Silesian Centre for Heart Diseases

Here, we describe a simple method that combines RNA fluorescence in situ hybridization (RNA-FISH) with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. This protocol may increase understanding of the molecular characteristics of SARS-CoV-2 RNA-host interactions at a single-cell level.

Chemistry

Microscopic Visualization of Porous Nanographenes Synthesized through a Combination of Solution and On-Surface Chemistry

Rafal Zuzak¹, Iago Pozo², Mads Engelund³, Manuel Vilas-Varela², José M. Alonso², Enrique Guitián², Dolores Pérez², Diego Peña², Szymon Godlewski¹

¹Centre for Nanometer-Scale Science and Advanced Materials, NANOSAM, Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, ²Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, ³Espeem S.A.R.L.

A combination of solution and surface assisted synthesis opens new directions in the atomically precise synthesis of nanostructures. Scanning tunneling microscopy (STM) supplemented by non-contact atomic force microscopy (nc-AFM) enables detailed characterization of newly designed and generated carbon-based nano-objects.

Medicine

Application of Lucilia sericata Larvae in Debridement of Pressure Wounds in Outpatient Settings

Dariusz Bazaliński^1,2, Paweł Więch², Paulina Szymańska³, Marek Muster², Maria Kózka⁴

¹Father B. Markiewicz Podkarpackie Specialist Oncology Centre, Specialist Hospital in Brzozów, ²Institute of Health Sciences, College of Medical Sciences, University of Rzeszów, ³General Surgery Clinic, Specialist Hospital in Radom, ⁴Department of Clinical Nursing, Faculty of Health Sciences, Collegium Medicum, Jagiellonian University

Maggots of Lucilia sericata were used in the debridement of deep pressure sores in outpatient settings for the treatment of pressure sores involving the complete skin thickness. The technique presented in the article does not pose risks for the patient.

Immunology and Infection

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Aneta Babula^*1,2, Izabela Siemińska^*1,3, Monika Baj-Krzyworzeka¹

¹Department of Clinical Immunology, Jagiellonian University Medical College, ²Doctoral School of Medical and Health Sciences, Jagiellonian University, ³Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU, University of Agriculture in Kraków

The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them.

Neuroscience

A Naturalistic Setup for Presenting Real People and Live Actions in Experimental Psychology and Cognitive Neuroscience Studies

Tuğçe Nur Pekçetin¹, Şeyda Evsen², Serkan Pekçetin³, Cengiz Acarturk⁴, Burcu A. Urgen⁵

¹Department of Cognitive Science, Middle East Technical University, ²Interdisciplinary Neuroscience Graduate Program, Bilkent University, ³Department of Computer Engineering, Middle East Technical University, ⁴Department of Cognitive Science, Jagiellonian University, ⁵Department of Psychology, Interdisciplinary Neuroscience Graduate Program, National Magnetic Resonance Research Center (UMRAM), Aysel Sabuncu Brain Research Center, Bilkent University

This study presents a naturalistic experimental setup that allows researchers to present real-time action stimuli, obtain response time and mouse tracking data while participants respond after each stimulus display, and change actors between experimental conditions with a unique system including a special transparent organic light-emitting diode (OLED) screen and light manipulation.