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Adam Mickiewicz University

2 ARTICLES PUBLISHED IN JoVE

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Chemistry

Surface Properties of Synthesized Nanoporous Carbon and Silica Matrices
Angelina Sterczyńska 1, Małgorzata Śliwińska-Bartkowiak 1,2, Małgorzata Zienkiewicz-Strzałka 3, Anna Deryło-Marczewska 3
1Faculty of Physics, Adam Mickiewicz University, 2NanoBioMedical Centre, 3Department of Physicochemistry of Solid Surface, Faculty of Chemistry, Maria Curie-Skłodowska University

Here we report the synthesis and characterization of ordered nanoporous carbon (with a 4.6 nm pore size) and SBA-15 (with a 5.3 nm pore size). The work describes the surface and textural properties of nanoporous molecular sieves, their wettability, and the melting behavior of D2O confined in the materials.

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Biology

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae
Deeksha Singh 1, Sara Blicharz 1, Karolina Stefanowicz 1, Laura Ragni 2, Kornel Michalak 3, Agnieszka Bagniewska-Zadworna 3, Robert Malinowski 1
1Integrative Plant Biology Team, Institute of Plant Genetics Polish Academy of Sciences, 2ZMBP-Center for Plant Molecular Biology, University of Tübingen, 3Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University

The present protocol describes an optimized method for the histological observation of galls induced by Plasmodiophora brassicae. Vibratome sections of hypocotyls are cleared before fluorescence imaging to study the involvement of transcription factors and phytohormones during disease progression. This protocol overcomes resin embedding limitations, enabling in planta visualization of fluorescent proteins.

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