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Wroclaw University of Science and Technology

1 ARTICLES PUBLISHED IN JoVE

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Engineering

Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction
Andrzej Żak 1, Olga Kaczmarczyk 2
1Electron Microscopy Laboratory, Faculty of Mechanical Engineering, Wroclaw University of Science and Technology, 2Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Science and Technology

The present protocol describes transmission electron microscope (TEM) modifications with a light illumination system, the fabrication of liquid cells, and in situ TEM observations of light-induced interactions between bacterial cells and a photosensitizer. The sample preparation methods, electron beam damage, and imaging are also discussed.

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