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Indiana University of Pennsylvania

3 ARTICLES PUBLISHED IN JoVE

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Biology

Transplantation of Cells Directly into the Kidney of Adult Zebrafish
Cuong Q. Diep 1, Alan J. Davidson 1
1Center for Regenerative Medicine, Massachusetts General Hospital

Cell transplantation is an essential technique for studying tissue regeneration and for developing cell-based therapies of disease. We demonstrate here a microsurgical technique that permits the transplantation of genetically labeled cells directly into the kidney of adult zebrafish fish.

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JoVE Core

Zebrafish as a Model to Assess the Teratogenic Potential of Nitrite
Vishal Keshari 1, Basma Adeeb 1, Alison E. Simmons 1, Thomas W. Simmons 1, Cuong Q. Diep 1
1Department of Biology, Indiana University of Pennsylvania

Exposure to teratogens may cause birth defects. Zebrafish are useful for determining the teratogenic potential of chemicals. We demonstrate the utility of zebrafish by exposing embryos to various levels of nitrite and also at different times of exposure. We show that nitrite can be toxic and cause severe developmental defects.

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Developmental Biology

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
Sagar N. Kasar 1, Sarah A. Grandinette 1, Scott D. Semelsberger 1, Cuong Q. Diep 1
1Department of Biology, Indiana University of Pennsylvania

The zebrafish is an important model for understanding kidney development. Here, an in situ hybridization protocol is optimized to detect gene expression in zebrafish larvae and juveniles during mesonephros development.

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