New South Wales Health Pathology

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Murray C. Killingsworth^1,2,3,4, Yuri V. Bobryshev^3,4,5

¹South Western Sydney Clinical School, Faculty of Medicine, University of New South Wales Australia, ²School of Medicine, Western Sydney University, ³Correlative Microscopy Group, Ingham Institute for Applied Medical Research, ⁴Electron Microscopy Laboratory, Department of Anatomical Pathology, Sydney South West Pathology Service, New South Wales Health Pathology, ⁵School of Medical Sciences, Faculty of Medicine, University of New South Wales Australia

A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.

Biology

Isolating Human Peripheral Blood Mononuclear Cells from Buffy Coats via High Throughput Immunomagnetic Bead Separation

Maria Villalva¹, Sara Macphail¹, Yingshi Li¹, Beth Caruana¹

¹New South Wales Health Statewide Biobank, New South Wales Health Pathology

This protocol details a high-throughput automation-compatible method to isolate human peripheral blood mononuclear cells for biobanking and other purposes.