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3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice
Yongli Zhang *1,2, Hangjie Zhang *2, Wenqiang Ma *3, Kefang Liu 1,2, Min Zhao 4, Yingze Zhao 2, Xuancheng Lu 5, Fuping Zhang 6, Xiangdong Li 3, George F. Gao 1,2,4,6, William J. Liu 1,2
1School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 2NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 3State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, 4Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, 5Laboratory Animal Center, Chinese Center for Disease Control and Prevention, 6CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences

A protocol to evaluate antigen-specific T-cell responses in the immunoprivileged organs of the Ifnar1-/- murine model for the Zika virus (ZIKV) infection is described. This method is pivotal for investigating the cellular mechanisms of the protection and immunopathogenesis of ZIKV vaccines and is also valuable for their efficacy evaluation.

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Immunology and Infection

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
Di Zhang *1,2, Kefang Liu *3,4, Dan Lu *2,5, Pengyan Wang 1,2, Qingxu Zhang 1,2, Peipei Liu 2, Yingze Zhao 2, Yan Chai 4, Jianxin Lyu 1, Jianxun Qi 4, William J. Liu 1,2
1School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 3Faculty of Health Sciences, University of Macau, 4CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 5Savaid Medical School, University of Chinese Academy of Sciences

The protocol describes experimental methods to obtain stable major histocompatibility complex (MHC) class I through potential β2-microglobulin (β2m) substitutions from different species. The structural comparison of MHC I stabilized by homologous and heterologous β2m were investigated.

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Immunology and Infection

Construction of Adenoviral Vectors using DNA Assembly Technology
Xiaohui Zou *1, Yun Zhu *2, Chenglong Li 1,3, Yali Duan 2, Linlin Zhang 2, Xiaojuan Guo 1, Wenzhe Hou 1, Zhengde Xie 2,4, Zhuozhuang Lu 1,5
1NHC Key Laboratory of Medical Virology and Viral Diseases, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 2Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Center for Children’s Health, 3Henan Chemical Technician College, 4Research Unit of Critical Infection in Childre, Chinese Academy of Medical Sciences, 5Chinese Academy of Sciences Joint Research Center for Emerging Infectious Diseases and Biosafety, Chinese Center for Disease Control and Prevention-Wuhan Institute of Virology

In this study, an infectious clone of human adenovirus type 7 (HAdV-7) was constructed, and an E3-deleted HAdV-7 vector system was established by modifying the infectious clone. This strategy used here can be generalized to make gene transfer vectors from other wild-type adenoviruses.

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