Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell BlocksShiv Poojan 1, Han-Seong Kim 2, Ji-Woon Yoon 1, Hye Won Sim 1, Kyeong-Man Hong 1
1Omics Core Lab, Research Institute, National Cancer Center, 2Department of Pathology, Inje University Ilsan Paik Hospital
Currently, immunofluorescent staining on fixed cells is the method of choice for determination of protein expression levels when morphological information is also necessary. This protocol presented herein provides for an alternative method of immunocytochemistry on paraffin-embedded cell blocks.
