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Norwegian Veterinary Institute

1 ARTICLES PUBLISHED IN JoVE

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Genetics

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Snorre Gulla 1, Saima Nasrin Mohammad 1, Duncan John Colquhoun 1,2
1Fish Health Research Group, Norwegian Veterinary Institute, 2University of Bergen

The Multi-Locus Variable-number tandem-repeat Analysis (MLVA) assay presented here enables inexpensive, robust and portable high-resolution genotyping of the fish-pathogenic bacterium Yersinia ruckeri. Starting from pure cultures, the assay employs multiplex PCR and capillary electrophoresis to produce ten-loci MLVA profiles for downstream applications.

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