Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted OrientationDoris A. Taylor 1, Luiz C. Sampaio 1, Rafael Cabello 1, Abdelmotagaly Elgalad 1, Rohan Parikh 1, R Patrick Wood 2, Kevin A. Myer 2, Alvin T. Yeh 3, Po-Feng Lee 1
1Regenerative Medicine Research, Texas Heart Institute, 2Lifegift Organ Donation Center, 3Biomedical Engineering, Texas A&M University
This method enables decellularization of a complex solid organ using a simple protocol based on osmotic shock and perfusion of ionic detergent with minimal organ matrix disruption. It comprises a novel decellularization technique for human hearts inside a pressurized pouch with real-time monitoring of flow dynamics and cellular debris outflow.