First Affiliated Hospital of Nanchang University

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Jing Wu¹, Roberta Pugh¹, Richard C. Laughlin¹, Helene Andrews-Polymenis², Michael McClelland³, Andreas J. Bäumler⁴, L. Garry Adams¹

¹Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, ²Department of Microbial and Molecular Pathogenesis, College of Medicine, Texas A&M University System Health Science Center, ³University of California, Irvine, ⁴Department of Medical Microbiology & Immunology, School of Medicine, University of California, Davis

A high-throughput assay to in vitro phenotype Salmonella or other bacterial association, invasion, and replication in phagocytic cells with high-throughput capacity was developed. The method was employed to evaluate Salmonella gene knockout mutant strains for their involvements in host-pathogen interactions.

Cancer Research

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Xiaoxing Lv¹, Meiru Zhao¹, Yuting Yi¹, Lucheng Zhang¹, Yanfang Guan¹, Tao Liu¹, Ling Yang¹, Rongrong Chen¹, Jianhui Ma¹, Xin Yi¹

¹Geneplus-Beijing Institute

This manuscript describes a technique for detecting mutations of low frequency in ctDNA, ER-Seq. This method is differentiated by its unique use of two-directional error correction, a special background filter, and efficient molecular acquirement.

Immunology and Infection

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

Jingshu Chen^*2, Lei Zhong^*1, Jing Wu¹, Sui Ke², Benjamin Morpurgo³, Andrei Golovko³, Nengtai Ouyang⁴, Yuxiang Sun², Shaodong Guo², Yanan Tian^1,2

¹Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agriculture University, ²Texas A&M University, ³Texas A&M Institute for Genomic Medicine, ⁴Sun Yat-Sen Memorial Hospital

Here we present a protocol to isolate mouse pancreatic islet cells for screening the ROS inductions by the xenobiotics in order to identify the potential diabetogenic xenobiotic chemicals.

Neuroscience

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

Mengye Zhu¹, Daying Zhang¹, Sicong Peng², Nana Liu², Jing Wu², Haixia Kuang², Tao Liu^2,3

¹Department of Pain, First Affiliated Hospital of Nanchang University, ²Department of Pediatrics, First Affiliated Hospital of Nanchang University, ³Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University

Here, we describe the essential steps for whole-cell patch-clamp recordings made from substantia gelatinosa (SG) neurons in the in vitro spinal cord slice. This method allows the intrinsic membrane properties, synaptic transmission and morphological characterization of SG neurons to be studied.

Medicine

OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery

Tao Liu^*1,2,3,4,5, Xueling Song^*1,2,3,4,5, Xiaoying Zheng^*1,2,3,4,5, Liying Yan^1,2,3,4,5, Caihong Ma^1,2,3,5, Rong Li^1,2,3,4,5, Jie Yan^1,2,3,4,5, Jie Qiao^1,2,3,4,5

¹Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, ²National Clinical Research Center for Obstetrics and Gynecology, ³Key Laboratory of Assisted Reproduction, Ministry of Education, ⁴Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, ⁵Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Chinese Academy of Medical Sciences

In vitro maturation (IVM) before gynecological operation (OP-IVM) combines IVM following oocyte retrieval with routine gynecological surgery and serves as an extension of conventional IVM applications for fertility preservation.