Quantification of Hypopigmentation Activity In VitroYeon-Ji Kim 1, Min-Jung Kim 1, Dong-Keon Kweon 2, Seung-Taik Lim 1, Sung-Joon Lee 1
1Department of Biotechnology, Graduate School of Life Sciences & Biotechnology, College of Life Sciences and Biotechnology, Korea University, 2JinWoo Bio Co. Ltd
We describe three experimental methods for evaluating the hypopigmentation activity of chemicals in vitro: quantification of 1) cellular tyrosinase activity and 2) melanin content, and 3) measurement of melanin by cellular melanin staining and image analysis.
