OVGU University of Magdeburg

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

Kerry Tedford¹, Laura Tech¹, Michael Steiner¹, Mark Korthals¹, Klaus-Dieter Fischer¹

¹Institute of Biochemistry and Cell Biology, OVGU University of Magdeburg

Marginal zone B cells (MZBs) respond to the force of shear flow by re-orienting their migration path up the flow. This protocol shows how to record and analyze the migration using a fluidics unit, pump, microscope imaging system, and free software.