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RIKEN Center for Brain Science

5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset
Misako Komatsu 1, Takaaki Kaneko 2,3, Hideyuki Okano 2,3, Noritaka Ichinohe 1,4
1Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science, 2Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, 3Department of Physiology, Keio University School of Medicine, 4Department of Ultrastructural Research, National Center of Neurology and Psychiatry

We have developed a whole-cortical electrocorticographic array for the common marmoset that continuously covers almost the entire lateral surface of cortex, from the occipital pole to the temporal and frontal poles. This protocol describes a chronic implantation procedure of the array in the epidural space of the marmoset brain.

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Neuroscience

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
Hiroko Bannai 1,2, Matsumi Hirose 2, Fumihiro Niwa 2,3, Katsuhiko Mikoshiba 2
1Japan Science and Technology Agency, PRESTO, 2Laboratory for Developmental Neurobiology, RIKEN Center for Brain Science, 3École Normale Supèrieure, Institut de Biologie de l'ENS (IBENS), Institut national de la santè et de la recherche mèdicale (INSERM), Centre national de la recherche scientifique (CNRS), École Normale Supèrieure, PSL Research University

Here we present a protocol for Ca2+ imaging in neurons and glial cells, which enables the dissection of Ca2+ signals at subcellular resolution. This process is applicable to all cell types that allow the expression of genetically encoded Ca2+ indicators.

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Neuroscience

Purification of the Dendritic Filopodia-rich Fraction
Yutaka Furutani 1,2, Yoshihiro Yoshihara 3
1Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, 2Liver Cancer Prevention Research Unit, RIKEN Center for Integrative Medical Sciences, 3Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science

In this protocol, we introduce a method for purifying the dendritic filopodia-rich fraction from the phagocytic cup-like protrusion structure on cultured hippocampal neurons by taking advantage of the specific and strong affinity between a dendritic filopodial adhesion molecule, TLCN, and an extracellular matrix molecule, vitronectin.

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Immunology and Infection

Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis
Davide Cossu 1,2, Yuji Tomizawa 1, Eiichi Momotani 1, Kazumasa Yokoyama 1, Nobutaka Hattori 1,3
1Department of Neurology, Juntendo University, 2Biomedical Research Core Facilities, Juntendo University, 3Neurodegenerative Disorders Collaborative laboratory, RIKEN Center for Brain Science

Here, we present an alternative protocol to actively induce experimental autoimmune encephalomyelitis in C57BL/6 mice, using the immunogenic epitope myelin oligodendrocyte glycoprotein (MOG)35-55 suspended in incomplete Freund's adjuvant containing the heat-killed Mycobacterium avium subspecies paratuberculosis.

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Medicine

Modification of the Treatment Methods for Wasting Marmoset Syndrome with Tranexamic Acid and Supportive Measures
Chie Sano 1, Ken Suenaga 1, Yuko Shigeno 1, Kimie Niimi 1
1Support Unit for Animal Resources Development, Research Resources Division, RIKEN Center for Brain Science

Here, we describe the modified treatment methods for wasting marmoset syndrome (WMS, also known as inflammatory bowel disease (IBD)-like disease) with tranexamic acid. We also present how to administer the therapeutic agents orally, subcutaneously, and intravenously.

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