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University of Nevada, Reno School of Medicine

4 ARTICLES PUBLISHED IN JoVE

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Biology

A Mouse Model of Intestinal Partial Obstruction
Se Eun Ha *1, Lai Wei *1, Brian G. Jorgensen *1, Moon Young Lee *2, Paul J. Park 1, Sandra M. Poudrier 1, Seungil Ro 1
1Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology, Wonkwang Digestive Disease Research Institute and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University

Intestinal obstructions are a partial or complete blockage of the intestine that can cause severe abdominal pain, nausea, vomiting, and preventing the passage of stool. This procedure for creating intestinal partial obsructions in mice is reliable in studying the mechanisms underlying pathological cell growth and death in the intestine.

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Biology

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
Bernard T. Drumm 1, Grant W. Hennig 2, Salah A. Baker 1, Kenton M. Sanders 1
1Department of Physiology and Cell Biology, University of Nevada Reno School of Medicine, 2Department of Pharmacology, The Robert Larner, M.D. College of Medicine, University of Vermont

Genetically encoded Ca2+ indicators (GECIs) have radically changed how in situ Ca2+ imaging is performed. To maximize data recovery from such recordings, appropriate analysis of Ca2+ signals is required. The protocols in this paper facilitate the quantification of Ca2+ signals recorded in situ using spatiotemporal mapping and particle-based analysis.

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Biology

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning
Nathan Grainger 1,2, Kenton M. Sanders 1, Bernard T. Drumm 1,3
1Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology, University of California School of Medicine, 3Department of Life & Health Sciences, Dundalk Institute of Technology

The goal of this protocol is to isolate intact pacemaker regions of the mouse renal pelvis using vibratome sectioning. These sections can then be used for in situ Ca2+ imaging to elucidate Ca2+ transient properties of pacemaker cells and other interstitial cells in vibratome slices.

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Neuroscience

Dosage-Adjusted Resistance Training in Mice with a Reduced Risk of Muscle Damage
Morium Begam 1, Neha Narayan 1, Drew Mankowski 1, Robert Camaj 1, Nicholas Murphy 1, Kevin Roseni 1, Marie E. Pepin 1, Jacob M. Blackmer 1, Takako I. Jones 2, Joseph A. Roche 1
1Physical Therapy Program, Department of Health Care Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 2Department of Pharmacology, University of Nevada, Reno School of Medicine

The present protocol describes a unique technique called dosage-adjusted resistance training (DART), which can be incorporated into precision rehabilitation studies performed in small animals, such as mice.

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