Research Institute of the McGill University Health Centre

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Snezana Vujicic^*1,2, Lanfei Feng^*1,2, Angelika Antoni³, Joyce Rauch⁴, Jerrold S. Levine^1,2,5

¹Section of Nephrology, Department of Medicine, University of Illinois at Chicago, ²Section of Nephrology, Department of Medicine, Jesse Brown Veterans Affairs Medical Center, ³Department of Biology, Kutztown University of Pennsylvania, ⁴Division of Rheumatology, Department of Medicine, Research Institute of the McGill University Health Centre, ⁵Department of Microbiology & Immunology, University of Illinois at Chicago

Here, we present a protocol for the determination of intracellular signaling events induced in viable cells by physical interaction with adjacent dead or dying cells. The protocol focuses on signaling events induced by receptor-mediated recognition of the dead cells, as opposed to their phagocytic uptake or release of soluble mediators.