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University Medical Center of the Johannes Gutenberg University Mainz

4 ARTICLES PUBLISHED IN JoVE

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Medicine

Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo
Adrian Gericke 1, Evgeny Goloborodko 1, Norbert Pfeiffer 1, Caroline Manicam 1
1Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz

Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.

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Biology

Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
Natarajan Perumal *1, Lars Straßburger 1, Carsten Schmelter 1, Adrian Gericke 1, Norbert Pfeiffer 1, Franz H. Grus 1, Caroline Manicam *1
1Department of Ophthalmology, University Medical Centre of the Johannes Gutenberg University Mainz

Proteome characterization of ocular microvascular beds is pivotal for in-depth understanding of many ocular pathologies in humans. This study demonstrates an effective, rapid, and robust method for protein extraction and sample preparation from small blood vessels employing the porcine short posterior ciliary arteries as model vessels for mass-spectrometry-based proteomics analyses.

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Neuroscience

Lipidomics and Transcriptomics in Neurological Diseases
Julia M. Post 1, Raissa Lerner 1, Claudia Schwitter 1, Beat Lutz 1, Ermelinda Lomazzo 1, Laura Bindila 1
1Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz

This article presents a modular protocol for tissue lipidomics and transcriptomics, and plasma lipidomics in neurological disease mouse models targeting lipids underlying inflammation and neuronal activity, membrane lipids, downstream messengers, and mRNA-encoding enzymes/receptors underlying lipid function. Sampling, sample processing, extraction, and quantification procedures are outlined.

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Neuroscience

Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function
Xufeng Chen 1, Danni Wang 1, Marcel Kegel 1, Jakob von Engelhardt 1
1Institute of Pathophysiology, University Medical Center of the Johannes Gutenberg University Mainz

Here, we present protocols for the preparation of acute brain slices containing the lateral geniculate nucleus and the electrophysiological investigation of retinogeniculate and corticogeniculate synapses function. This protocol provides an efficient way to study synapses with the high-release and low-release probability in the same acute brain slices.

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