Creighton University School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Genetics

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

¹Department of Medical Microbiology and Immunology, Creighton University School of Medicine

This molecular-based approach for determining bacterial fitness facilitates precise and accurate detection of microorganisms using unique genomic DNA barcodes that are quantified via digital PCR. The protocol describes calculating the competitive index for Salmonella strains; however, the technology is readily adaptable to protocols requiring absolute quantification of any genetically-malleable organism.

Biology

Transfection of a Molecular Clone of Naegleria gruberi rDNA into N. gruberi Trophozoites

Brian T. Nguyen¹, Nora M. Chapman², John C. Mullican³, Kristen M. Drescher¹

¹Department of Medical Microbiology and Immunology, Creighton University School of Medicine, ²Department of Pathology and Microbiology, University of Nebraska Medical Center, ³Department of Biology, Washburn University

This protocol describes a methodology to transfect Naegleria gruberi trophozoites with a construct that is maintained throughout passaging trophozoites in vitro, as well as through encystment and excystment.