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University of Bremen

5 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging
Maria Lucia Pigazzini *1,2, Christian Gallrein *1, Manuel Iburg *1, Gabriele Kaminski Schierle 3, Janine Kirstein 1,4
1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin, 2NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, 3Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology, University of Cambridge, 4Cell Biology, University of Bremen

Fluorescence lifetime imaging monitors, quantifies and distinguishes the aggregation tendencies of proteins in living, aging, and stressed C. elegans disease models.

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Behavior

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice
Laura Berg 1,2, Jill Gerdey 1,2, Olivia A. Masseck 1,2
1Biology and Chemistry, Synthetic Biology, University of Bremen, 2Advanced Fluorescence Microscopy, Faculty of Biology and Biotechnology, Ruhr-Universität Bochum

With optogenetic manipulation of specific neuronal populations or brain regions, behavior can be modified with high temporal and spatial resolution in freely moving animals. By using different optogenetic tools in combination with chronically implanted optical fibers, a variety of neuronal modulations and behavioral testing can be performed.

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Medicine

Semi-Minimal Invasive Method to Induce Myocardial Infarction in Rats and the Assessment of Cardiac Function by an Isolated Working Heart System
Patrick M. Pilz 1, Miriam Lang 1, Ouafa Hamza 1, Petra L. Szabo 1, Milat Inci 1, Anne M. Kramer 1, Michael Koch 2, Johann Huber 3, Bruno K. Podesser 1, Attila Kiss 1
1Ludwig-Boltzmann-Institute for Cardiovascular Research at the Center for Biomedical Research, Medical University of Vienna, 2Concept & Ideation, Electrical Sector/EMEA/Power Distribution Division, Eaton Industries (Austria) GmbH, 3Vetfarm and Clinical Unit for Herd Health Management in Ruminants, Clinic for Ruminants, Vetmeduni Vienna

This article presents an efficient method to perform myocardial ischemia and subsequent chronic reperfusion in rats using a minimally invasive approach. In addition, left ventricular hemodynamic function of rats is assessed by echocardiography and isolated working heart methods. 

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Biology

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2
Maria Lucia Pigazzini 1,2, Janine Kirstein 1,3
1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund, 2NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, 3Department of Biology and Chemistry, University of Bremen

Presented here is a protocol to monitor degradation of the protein huntingtin fused to the photoconvertible fluorophore Dendra2.

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Behavior

An Open-Source, Fully Customizable 5-Choice Serial Reaction Time Task Toolbox for Automated Behavioral Training of Rodents
Julia Morais Gancz 1, Nada El Jundi 2, Eva Strippelmann 2, Michael Koch 2, Detlef Wegener 1
1Cognitive Neurophysiology Department, Brain Research Institute, University of Bremen, 2Neuropharmacology Department, Brain Research Institute, University of Bremen

The present protocol describes the development of an open-source 5-choice serial reaction time task toolbox for rodent animal models, using Arduino and related hardware and a versatile Matlab toolbox, including an optional script for automated behavioral training. The scripts are customizable and facilitate the implementation of different trial-and-test designs.

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