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University of the Pacific

7 ARTICLES PUBLISHED IN JoVE

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Biology

Microdissection of Black Widow Spider Silk-producing Glands
Felicia Jeffery *1, Coby La Mattina *1, Tiffany Tuton-Blasingame *1, Yang Hsia 1, Eric Gnesa 1, Liang Zhao 1, Andreas Franz 1, Craig Vierra 1
1Department of Biological Sciences, University of the Pacific

Here we describe an efficient strategy to remove the silk-producing glands from the abdomen of female black widow spiders. This procedure allows the rapid isolation of the seven distinct silk-producing glands in a highly purified fashion, an important process for investigators studying spider silk production and fiber assembly.

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Bioengineering

Synthetic Spider Silk Production on a Laboratory Scale
Yang Hsia 1, Eric Gnesa 1, Ryan Pacheco 1, Kristin Kohler 1, Felicia Jeffery 1, Craig Vierra 1
1Department of Biological Sciences, University of the Pacific

Despite the outstanding mechanical and biochemical properties of spider silks, this material cannot be harvested in large quantities by conventional means. Here we describe an efficient strategy to spin artificial spider silk fibers, which is an important process for investigators studying spider silk production and their use as next-generation biomaterials.

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Chemistry

Determination of the Gas-phase Acidities of Oligopeptides
Jianhua Ren 1, Ashish Sawhney 1, Yuan Tian 1, Bhupinder Padda 1, Patrick Batoon 1
1Department of Chemistry, University of the Pacific

The determination of the gas-phase acidities of cysteine-containing oligopeptides is described. The experiments are performed using a triple quadrupole mass spectrometer. The relative acidities of the peptides are measured using collision-induced dissociation experiments, and the quantitative acidities are determined using the extended Cooks kinetic method.

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Behavior

Infant Auditory Processing and Event-related Brain Oscillations
Gabriella Musacchia 1,2,3, Silvia Ortiz-Mantilla 1, Teresa Realpe-Bonilla 1, Cynthia P. Roesler 1, April A. Benasich 1
1Center for Molecular & Behavioral Neuroscience, Rutgers University, State University of New Jersey, Newark, 2Department of Audiology, University of the Pacific, 3Department of Otolaryngology, Head & Neck Surgery, Stanford University

High-density electroencephalography (dEEG) is being used increasingly to study brain development and plasticity in the early years of life. Here we present an application of sophisticated analysis techniques that builds on traditional EEG recording to understand the oscillatory dynamics of rapid auditory processing in the infant brain.

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Chemistry

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids
Jianhua Ren 1, Yadwinder S. Mann 1, Yuntao Zhang 1, Michael D. Browne 1
1Department of Chemistry, University of the Pacific

A protocol is described for the manual synthesis of oligo-peptoids followed by sequence analysis by mass spectrometry.

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Medicine

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model
Yuan Yuan 1, Meng-hua Chen 1, Jing Huang 2, Yuan Tian 2, Ke Qin 3, Zhang Yuan 1, Wen-yan Wang 1, Zhi-jiang Wu 1, Xin-yue Tian 1, Yubin Zhang 4
1Department of Intensive Care Unit, The Second Affiliated Hospital of Guangxi Medical University, 2Department of hepatobiliary surgery, The Affiliated Ningbo Medical Center Lihuili Hospital of Medical School of Ningbo University, 3Department of transplantation, The Second Affiliated Hospital of Guangxi Medical University, 4Digital Technology and Engineering college, Ningbo University of Finance & Economics

This paper provides a detailed description of how to build an animal model of the anhepatic phase (liver ischemia) in rats to facilitate basic research into ischemia-reperfusion injury after liver transplantation.

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Cancer Research

Construction of An Orthotopic Xenograft Model of Non-Small Cell Lung Cancer Mimicking Disease Progression and Predicting Drug Activities
Zizhao Xu 1, Yingbo Huang 1, Yong Zhu 1, Xinyu Pei 1, Yifan Lu 1, Shen Zhao 1, Xin Guo 1
1Thomas J. Long School of Pharmacy, University of the Pacific

This study presents an orthotopic non-small cell lung cancer (NSCLC) model based on intrapulmonary inoculation of multicellular spheroids of fluorescent A549-iRFP cells. The model recapitulates clinical NSCLC stages and responds to cisplatin, according to dynamic in vivo monitoring of long-wavelength fluorescence.

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