Hospital de la Santa Creu I Sant Pau

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma

Natalia Valle-Tamayo^1,2, Rocío Pérez-González^2,3, Gemma Chiva-Blanch^4,5, Olivia Belbin^1,2, Sara Serrano-Requena^1,2, Sònia Sirisi^1,2, Alba Cervantes González^1,2, Oriol Giró^4,5, Érika Sánchez-Aced^1,2, Oriol Dols-Icardo^1,2, Daniel Alcolea^1,2, María Carmona-Iragui^1,2, Amanda Jimenez^4,5, Alberto Lleó^1,2, Juan Fortea^1,2, M. Florencia Iulita^1,2

¹Sant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau, Universitat Autònoma de Barcelona, ²Center of Biomedical Investigation Network for Neurodegenerative Diseases (CIBERNED), ³Movement Disorders Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, ⁴Department of Endocrinology and Nutrition, Hospital Clínic de Barcelona, August Pi i Sunyer Biomedical Research Institute - IDIBAPS, ⁵Spanish Biomedical Research Network in Physiopathology of Obesity and Nutrition (CIBEROBN)

This protocol describes the enrichment of astrocyte-derived extracellular vesicles (ADEVs) from human plasma. It is based on the separation of EVs by polymer precipitation, followed by ACSA-1 based immunocapture of ADEVs. Analysis of ADEVs may offer clues to study changes in inflammatory pathways of living patients, non-invasively by liquid biopsy.

Medicine

Popliteal Vascular Lymph Node Resection in the Rabbit Hindlimb for Secondary Lymphedema Induction

José Luis Campos¹, Gemma Pons², Elena Rodriguez², Ali M. Al-Sakkaf², Francisco Javier Vela¹, Laura Pires¹, María Jesús Jara¹, Francisco Miguel Sánchez-Margallo¹, Elena Abellán¹, Jaume Masiá²

¹Department of Microsurgery, Jesús Usón Minimally Invasive Surgery Centre, ²Department of Plastic Surgery, Hospital de la Santa Creu i Sant Pau

Here, the surgical induction of stable acquired lymphedema in the rabbit hindlimb is described. This experimental animal can be used to further investigate the effect of lymphedema treatment by microsurgical techniques.