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Tennessee State University

6 ARTICLES PUBLISHED IN JoVE

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Biology

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Chen Lu 1, Joshua L. Wonsidler 1, Jianwei Li 2, Yanming Du 1, Timothy Block 3, Brian Haab 4, Songming Chen 1
1Institute for Hepatitis and Virus Research, 2Department of Microbiology and Immunology, Thomas Jefferson University, 3Drexel University College of Medicine, 4Van Andel Research Institute

In this study, we describe an improved protocol for a multiplexed high-throughput antibody microarray with lectin detection method that can be used in glycosylation profiling of specific proteins. This protocol features new reliable reagents and significantly reduces the time, cost, and lab equipment requirements as compared to the previous procedure.

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Biology

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen
Yizhong Huang 1, Maozhang He 1, Qingjie Zeng 1, Lin Li 1, Zhen Zhang 1, Junwu Ma 1, Yanyu Duan 1
1State Key Laboratory for Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University

This protocol describes a procedure to freeze muscle tissues by plunging them directly into liquid nitrogen. This protocol also highlights a new cryovial that can avoid the "blanket effect" of nitrogen gas when liquid nitrogen contacts the tissue surface of a specimen.

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JoVE Journal

Sampling Soils in a Heterogeneous Research Plot
Jianwei Li 1
1Department of Agriculture and Environmental Science, Tennessee State University

The traditional soil-sampling procedure determines the number of soil samples arbitrarily. Here, we provide a simple yet efficient clustered soil-sampling design to demonstrate soil spatial heterogeneity and quantitatively determine the number of soil samples required and the associated sampling accuracy.

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Engineering

Sandy Soil Improvement through Microbially Induced Calcite Precipitation (MICP) by Immersion
Shihui Liu 1, Kang Du 1, Kejun Wen 1, Wei Huang 2, Farshad Amini 1, Lin Li 3
1Department of Civil & Environmental Engineering, Jackson State University, 2School of Civil Engineering and Architecture, Chongqing University of Science and Technology, 3Department of Civil & Architectural Engineering, Tennessee State University

Here, microbially induced calcite precipitation (MICP) technology is presented to improve soil properties by immersion.

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Environment

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
Owen Hudson 1, Sumyya Waliullah 1, Justin Hand 2, Romina Gazis-Seregina 3, Fulya Baysal-Gurel 4, Md Emran Ali 1
1Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, 2University of Georgia Cooperative Extension, 3Department of Plant Pathology, Tropical Research & Education Center, University of Florida, 4Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University

We developed a method to detect Phytophthora capsici zoospores in water sources using a filter paper DNA extraction method coupled with a loop-mediated isothermal amplification (LAMP) assay that can be analyzed in the field or in the lab.

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Environment

Simulating Temperature in a Soil Incubation Experiment
Jianwei Li 1, Precious Areeveso 1, Xuehan Wang 1, Siyang Jian 1,2, Lahiru Gamage 1
1Department of Agricultural and Environmental Sciences, Tennessee State University, 2Department of Plant Biology and Microbiology, University of Oklahoma, Norman

Laboratory soil warming experiments usually employ two or more constant temperatures in multiple chambers. By presenting a sophisticated environmental chamber, we provide an accurate temperature control method to imitate the magnitude and amplitude of in situ soil temperature and improve the experimental design of soil incubation studies.

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