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Claremont McKenna College

3 ARTICLES PUBLISHED IN JoVE

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Engineering

Drawing and Hydrophobicity-patterning Long Polydimethylsiloxane Silicone Filaments
Katherine Snell 1, Isabelle Lopez 2, Brandon Louie 1, Roxanna Kiessling 1, Babak Sanii 1,2,3
1Keck Science Department, Claremont McKenna College, 2Keck Science Department, Scripps College, 3Keck Science Department, Pitzer College

Here, we present a protocol to produce long filaments of polydimethylsiloxane (PDMS) silicone by gravity-drawing through a furnace. Filaments are on the order of hundreds of micrometers in diameter and tens of centimeters in length and are hydrophobically patternable via an Arduino-controlled corona discharge system.

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Neuroscience

Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections
Yuu Ohno 1, Riley Murphy 2, Matthew Choi 3, Weijun Ou 4, Rachita K. Sumbria 4,5
1Henry E. Riggs School of Applied Life Sciences, Keck Graduate Institute, 2Crean College of Health and Behavioral Sciences, Chapman University, 3Keck Science Department, Claremont McKenna College, 4Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy, Chapman University, 5Department of Neurology, University of California, Irvine

The present protocol describes and compares the procedure to perform a full-region or sub-region of interest analysis of sagittal mouse brain sections to quantify amyloid-beta load in the APP/PS1 transgenic mouse model of Alzheimer's disease.

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Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks
Nathan Felcher 1, Daisy Achiriloaie 1, Brian Lee 2, Ryan McGorty 3, Janet Sheung 1,2,4
1W.M. Keck Science Department, Scripps College, 2W.M. Keck Science Department, Claremont McKenna College, 3Department of Physics and Biophysics, University of San Diego, 4W.M. Keck Science Department, Pitzer College

This protocol describes in detail how to build a single-objective, light-sheet fluorescence microscope and its usage for visualizing cytoskeleton networks.

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