Claremont McKenna College

1 ARTICLES PUBLISHED IN JoVE

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks

Nathan Felcher¹, Daisy Achiriloaie¹, Brian Lee², Ryan McGorty³, Janet Sheung^1,2,4

¹W.M. Keck Science Department, Scripps College, ²W.M. Keck Science Department, Claremont McKenna College, ³Department of Physics and Biophysics, University of San Diego, ⁴W.M. Keck Science Department, Pitzer College

This protocol describes in detail how to build a single-objective, light-sheet fluorescence microscope and its usage for visualizing cytoskeleton networks.