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Wageningen University & Research

7 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
Titis A.K. Wardhani 1,2, Yuda Purwana Roswanjaya 1,2, Simon Dupin 1,3, Huchen Li 1,4, Sidney Linders 1, Marijke Hartog 1, Rene Geurts 1, Arjan van Zeijl 1
1Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, 2Center of Technology for Agricultural Production, Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture

Parasponia andersonii is a fast-growing tropical tree that belongs to the Cannabis family (Cannabaceae) and can form nitrogen-fixing root nodules in association with the rhizobium. Here, we describe a detailed protocol for reverse genetic analyses in P. andersonii based on Agrobacterium tumefaciens-mediated stable transformation and CRISPR/Cas9-based genome editing.

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Biology

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T
Remco van Schadewijk 1, Julia R. Krug 2,3,4, Andrew Webb 5, Henk Van As 2,4, Aldrik H. Velders 3,4, Huub J. M. de Groot 1, A. Alia 1,6
1Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science, Leiden University, 2Laboratory of Biophysics, Wageningen University & Research, 3Laboratory of BioNanoTechnology, Wageningen University & Research, 4MAGNEtic resonance Facility, Wageningen University & Research, 5C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre, Leiden University, 6Institute for Medical Physics and Biophysics, Leipzig University

A protocol to study biological tissue at high spatial resolution using ultra-high field magnetic resonance microscopy (MRM) using microcoils is presented. Step-by-step instructions are provided for characterizing the microcoils. Finally, optimization of imaging is demonstrated on plant roots.

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Behavior

Iterative Development of an Innovative Smartphone-Based Dietary Assessment Tool: Traqq
Desiree A. Lucassen 1, Elske M. Brouwer-Brolsma 1, Anne M. van de Wiel 1, Els Siebelink 1, Edith J. M. Feskens 1
1Division of Human Nutrition and Health, Wageningen University and Research

This article describes the protocol for the development of an innovative smartphone-based dietary assessment application Traqq, including expert evaluations and usability testing.

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JoVE Core

Concept Development and Use of an Automated Food Intake and Eating Behavior Assessment Method
Marlou P. Lasschuijt 1, Elske Brouwer-Brolsma 1, Monica Mars 1, Els Siebelink 1, Edith Feskens 1, Kees de Graaf 1, Guido Camps 1
1Division of Human Nutrition and Health, Wageningen University & Research

This protocol shows and explains a new technology-based dietary assessment method. The method consists of a dining tray with multiple built-in weighing scales and a video camera. The device is unique in the sense that it incorporates automated measures of food and drink intake and eating behavior over the course of a meal.

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Bioengineering

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
Chang Chen *1, Ketan A. Ganar *1, Siddharth Deshpande 1
1Laboratory of Physical Chemistry and Soft Matter, Wageningen University & Research

The present protocol describes octanol-assisted liposome assembly (OLA), a microfluidic technique to generate biocompatible liposomes. OLA produces monodispersed, micron-sized liposomes with efficient encapsulation, allowing immediate on-chip experimentation. This protocol is anticipated to be particularly suitable for synthetic biology and synthetic cell research.

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Engineering

Real-Time Imaging of Bonding in 3D-Printed Layers
J. Jesse Buijs 1, Raoul Fix 1, Hanne M. van der Kooij 1, Thomas E. Kodger 1
1Physical Chemistry and Soft Matter, Wageningen University & Research

With a non-invasive and real-time technique, nanoscopic polymer motion inside a polymer filament is imaged during 3D printing. Fine-tuning this motion is crucial for producing constructs with optimal performance and appearance. This method reaches the core of plastic layer fusion, thus offering insights into optimal printing conditions and material design criteria.

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Biochemistry

Design and Construction of an Experimental Setup to Enhance Mineral Weathering through the Activity of Soil Organisms
Tullia Calogiuri 1,2, Mathilde Hagens 2, Jan Willem Van Groenigen 1, Thomas Corbett 3, Jens Hartmann 4, Rick Hendriksen 5, Iris Janssens 6, Ivan A. Janssens 7, Guillermo Ledesma Dominguez 7, Grant Loescher 4, Steven Mortier 6, Anna Neubeck 3, Harun Niron 7, Reinaldy P. Poetra 4, Lukas Rieder 4, Eric Struyf 7, Michiel Van Tendeloo 8, Tom De Schepper 6, Tim Verdonck 9, Siegfried E. Vlaeminck 8, Sara Vicca 7, Alix Vidal 1
1Soil Biology Group, Wageningen University & Research, 2Soil Chemistry and Chemical Soil Quality, Wageningen University & Research, 3Department of Earth Sciences, Uppsala University, 4Institute for Geology, Center for Earth System Research and Sustainability, University of Hamburg, 5Tupola, Wageningen University & Research, 6IDLab - Department of Computer Science, University of Antwerp - imec, 7Plants and Ecosystems (PLECO), Biology Department, University of Antwerp, 8Research Group of Sustainable Energy, Air and Water Technology, University of Antwerp, 9Department of Mathematics, University of Antwerp - imec

Here we present the construction and operation of an experimental setup to enhance mineral weathering through the activity of soil organisms while concurrently manipulating abiotic variables known to stimulate weathering. Representative results from the functioning of the setup and sample analyses are discussed together with points for improvement.

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