Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical InteractionsQuentin Fuchs 1, Aurélie Batut 2, Mélanie Gleyzes 2, Jessica Rontard 2, Louise Miny 2, Margot Libralato 2, Janaina Vieira 2, Delphine Debis 2, Florian Larramendy 2, Thibault Honegger 2, Melissa Messe 1, Marina Pierrevelcin 1, Benoit Lhermitte 1,3, Monique Dontenwill 1, Natacha Entz-Werlé 1,4
1Team Tumoral signaling and therapeutic targets, UMR CNRS 7021 - Laboratory of Bioimaging and Pathologies, 2NETRI, 3Centre de Ressources Biologiques, Pathology department, University Hospital of Strasbourg, 4Pediatric Oncohematology unit, University Hospital of Strasbourg
Recent works uncover the neuronal impact on high-grade pediatric glioma (pHGG) cells and their reciprocal interactions. The present work shows the development of an in vitro model co-culturing pHGG cells and glutamatergic neurons and recorded their electrophysiological interactions to mimic those interactivities.
