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3 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
Ziming Cheng 1, Ting Zhou 1,2, Azhar Merchant 1, Thomas J. Prihoda 3, Brian L. Wickes 4,5, Guogang Xu 2, Christi A. Walter 2,5, Vivienne I. Rebel 1,2,5
1Greehey Children's Cancer Research Institute, UT Health Science Center at San Antonio, 2Department of Cellular and Structural Biology, UT Health Science Center at San Antonio, 3Department of Pathology, UT Health Science Center at San Antonio, 4Department of Microbiology, UT Health Science Center at San Antonio, 5Cancer Therapy and Research Center, UT Health Science Center at San Antonio

Here we describe an in vivo mutagenesis assay for small numbers of purified hematopoietic cells using the LacI transgenic mouse model. The LacI gene can be isolated to determine the frequency, location, and type of DNA mutants spontaneously arisen or after exposure to genotoxins.

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JoVE Core

Quality-Controlled Sputum Analysis by Flow Cytometry
Marcia Grayson *1, Shao-Chiang Lai *1, Lydia H. Bederka 1, Patricia Araujo 1, Jamila Sanchez 1, Xavier T. Reveles 1, Vivienne I. Rebel 1,2, Jennifer Rebeles 1
1bioAffinity Technologies, 2Department of Cell Systems & Anatomy, The University of Texas Health Science Center at San Antonio

This protocol describes an efficient method for dissociating sputum into a single cell suspension and the subsequent characterization of cellular subsets on standard flow cytometric platforms.

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Chemistry

Porphyrin-Modified Beads for Use as Compensation Controls in Flow Cytometry
William Bauta 1, Marcia Grayson 1, Rossella Titone 1, Jennifer Rebeles 1, Vivienne I. Rebel 1
1bioAffinity Technologies

The protocol describes how porphyrin-based compensation beads for flow cytometry are prepared by the reaction of amine-functionalized polystyrene beads with the porphyrin TCPP and the amide coupling reagent EDC. A filtration procedure is used to reduce the particulate byproducts.

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