Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline GliomaGiulia Pericoli *1, Roberta Ferretti *1, Andrew S. Moore 2,3, Maria Vinci 1
1Department of Onco-hematology, Gene and Cell Therapy, Bambino Gesù Children's Hospital, IRCCS, 2Oncology Service, Children's Health Queensland Hospital and Health Service, 3Child Health Research Centre, The University of Queensland
This study presents a protocol of live-3D-cell immunocytochemistry applied to a pediatric diffuse midline glioma cell line, useful to study in real-time the expression of proteins on the plasma membrane during dynamic processes like 3D cell invasion and migration.
