Optimizing Extracellular Vesicle Delivery Using a Core-Sheath 3D-Bioprinted Scaffold for Chronic Wound ManagementSaeid Vakilian 1,2, Fatemeh Jamshidi-adegani 1, Fahad Al-Fahdi 1, Juhaina Al-Kindi 1, Ahmed Al-Harrasi 1, Sulaiman Al-Hashmi 1
1Laboratory for Stem Cell and Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, 2Doctoral Programme in Biomedicine, Faculty of Medicine, University of Helsinki
This study presents a protocol for fabricating core-sheath 3D-bio-printed scaffolds for chronic wound healing. Extracellular vesicles are isolated from mesenchymal stem cells, and loaded into the core (alginate) with the sheath made from carboxymethyl cellulose and alginate lyase. This design allows controlled scaffold degradation and efficient EVs release.
