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University of Belgrade Faculty of Biology

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
Katarina Milićević 1, Andrej Korenić 1, Milena Milošević 1, Pavle R. Andjus 1
1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”, University of Belgrade Faculty of Biology

We present here a protocol on how to prepare primary cultures of glial cells, astrocytes, and microglia from rat cortices for time-lapse video imaging of intracellular Ca2+ for research on pathophysiology of amyotrophic lateral sclerosis in the hSOD1G93A rat model.

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