JoVE Logo

Sign In

Aptagen LLC

2 ARTICLES PUBLISHED IN JoVE

image

Biochemistry

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
Tran Thi Thanh Thoa 1, Albert M. Liao 1, G. Thomas Caltagirone 1
1Aptagen LLC

The present protocol describes the use of Isothermal Titration Calorimetry (ITC) to analyze the association and dissociation kinetics of the binding between a DNA aptamer and tetracycline, including sample preparation, running standards and samples, and interpreting the resulting data.

image

Bioengineering

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Albert M. Liao 1, Tran Thi Thanh Thoa 1, G. Thomas Caltagirone 1
1Aptagen LLC

The present protocol demonstrates the use of a fast, 3-stage, aptamer-based exponential amplification assay to detect targets. Sample preparation, signal amplification, and color development are covered to implement this system to recognize the presence of theophylline over that of caffeine.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved