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North Dakota State University

4 ARTICLES PUBLISHED IN JoVE

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Biology

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm
Aurelia C. Kucera 1, Britt J. Heidinger 2
1Environmental and Conservation Sciences Program, North Dakota State University, 2Department of Biological Sciences, North Dakota State University

This protocol describes cloacal massage, a non-invasive manual technique for collecting semen from birds, and DNA extraction from avian sperm using a commercial extraction kit with modifications.

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Environment

A Precise and Autonomous System for the Detection of Insect Emergence Patterns
Meghan M. Bennett 1,2, Joseph P. Rinehart 3, George D. Yocum 3, Ian Yocum 3
1Department of Biological Sciences, North Dakota State University, 2School of Life Sciences, Arizona State University, 3Red River Valley Agricultural Research Center, USDA-ARS

Measurement of insect emergence patterns requires precision. Existing systems are only semi-automated and sample size is limited. We addressed these issues by designing a system using microcontrollers to precisely measure the time of emergence of large numbers of emerging insects.

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Bioengineering

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
Meihua Wan 1,2, Pouya Amrollahi 2,3, Dali Sun 4, Christopher Lyon 2,3, Tony Y. Hu 2,3
1Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, 2Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute, Arizona State University, 3School of Biological and Health Systems Engineering, Arizona State University, 4Department of Electrical and Computer Engineering, North Dakota State University

Clinical translation of exosome-derived biomarkers for diseased and malignant cells is hindered by the lack of rapid and accurate quantification methods. This report describes the use of low-magnification dark-field microscope images to quantify specific exosome subtypes in small volume serum or plasma samples.

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Bioengineering

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds
Bharat Kumar Reddy Sanapalli 1, Kalaivani Chinna Gounder 1, Nilesh Sudhakar Ambhore 2, Gowthamarajan Kuppuswamy 1, Praveen Thaggikuppe Krishnamurthy 3, Veera Venkata Satyanarayana Reddy Karri 1
1Department of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education & Research, 2Department of Pharmaceutical Sciences, School of Pharmacy, North Dakota state university, 3Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education & Research

The prepared DOX-CL scaffold satisfied the prerequisites of an ideal DW dressing in mechanical strength, porosity, water absorption, degradation rate, sustained release, anti-bacterial, biocompatibility, and anti-inflammatory properties, which are considered to be essential for the recovery of damaged tissue in DWs.

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