SUNY-University at Buffalo

4 ARTICLES PUBLISHED IN JoVE

Biology

In situ Protocol for Butterfly Pupal Wings Using Riboprobes

¹Department of Biological Sciences, SUNY-University at Buffalo, ²Dept. Ecology and Evolutionary Biology, Yale University

In order to examine gene expression in the pupal wing tissue of Bicyclus anynana, we present an optimized protocol for in situ hybridizations using riboprobes. We also provide guidelines for the further optimization of this protocol for use in pupal wings of other Lepidopteran species.

Neuroscience

Visualization of Larval Segmental Nerves in 3^rd Instar Drosophila Larval Preparations

Samantha Fye¹, Kunsang Dolma¹, Min Jung Kang¹, Shermali Gunawardena¹

¹Department of Biological Sciences, SUNY-University at Buffalo

Drosophila melanogaster larvae provide an ideal model system to investigate the mechanisms of axonal transport within larval segmental nerves. Using this procedure, 3^rd instar larvae carrying various mutations can be compared to wild type larvae.

Neuroscience

Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos

Tadeusz J. Kaczynski¹, Shermali Gunawardena¹

¹Department of Biological Sciences, SUNY-University at Buffalo

We describe the procedure to prepare staged Drosophila embryos for the visualization of the embryonic nervous system during embryogenesis.

Neuroscience

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

Michelle L. Kuznicki¹, Shermali Gunawardena¹

¹Department of Biological Sciences, SUNY-University at Buffalo

This protocol discusses the live dissection of Drosophila larvae for the purpose of imaging the movement of GFP tagged axonal vesicles on microtubule tracks.