Chromatin Immunoprecipitation from Human Embryonic Stem CellsStephane Bertani 1, Alice Kan 1, Frank Sauer 1
1Department of Biochemistry, University of California - Riverside
The differentiation of ESC coincides with cell-type specific changes in the structure and composition of chromatin. The detection of those changes provides valuable insights into the mechanisms that define stemcellness and cell differentiation. Chromatin immunoprecipitation (ChIP) represents a valuable method to dissect the molecular mechanisms underlying stem cell differentiation.