JoVE Logo

Sign In

University of Birmingham

4 ARTICLES PUBLISHED IN JoVE

image

Biology

Reaggregate Thymus Cultures
Andrea White 1, Eric Jenkinson 1, Graham Anderson 1
1MRC Center for Immune Regulation, University of Birmingham

In this video the preparation of 2-dGuo-treated reaggregate thymus cultures is demonstrated.

image

Biology

Preparation of 2-dGuo-Treated Thymus Organ Cultures
William Jenkinson 1, Eric Jenkinson 1, Graham Anderson 1
1MRC Center for Immune Regulation, University of Birmingham

This video demonstrates the dissection and removal of the fetal thymus as well the preparation of ex vivo cultures of 2-dGuo-treated thymus.

image

Medicine

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
Kenneth M. Tichauer 1, Robert W. Holt 2, Kimberley S. Samkoe 3, Fadi El-Ghussein 1, Jason R. Gunn 1, Michael Jermyn 1, Hamid Dehghani 4, Frederic Leblond 1, Brian W. Pogue 1,2
1Thayer School of Engineering, Dartmouth College, 2Department of Physics and Astronomy, Dartmouth College, 3Darmouth Medical School, Dartmouth College, 4School of Computer Science, University of Birmingham

Diffuse fluorescence tomography offers a relatively low-cost and potentially high-throughout approach to preclinical in vivo tumor imaging. The methodology of optical data collection, calibration, and image reconstruction is presented for a computed tomography-guided non-contact time-domain system using fluorescent targeting of the tumor biomarker epidermal growth factor receptor in a mouse glioma model.

image

Neuroscience

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila
Kentaro Kato 1, Alicia Hidalgo 1
1Neurodevelopment Group, School of Biosciences, University of Birmingham

An injury paradigm using the Drosophila larval ventral nerve cord to investigate central nervous system regeneration and repair is described. Stabbing followed by laser scanning confocal microscopy in time-lapse and fixed specimens, combined with quantitative analysis with purposefully developed software and genetics, are used to investigate the molecular mechanisms of CNS regeneration and repair.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved