University of Birmingham

4 ARTICLES PUBLISHED IN JoVE

Biology

Reaggregate Thymus Cultures

Andrea White¹, Eric Jenkinson¹, Graham Anderson¹

¹MRC Center for Immune Regulation, University of Birmingham

In this video the preparation of 2-dGuo-treated reaggregate thymus cultures is demonstrated.

Biology

Preparation of 2-dGuo-Treated Thymus Organ Cultures

William Jenkinson¹, Eric Jenkinson¹, Graham Anderson¹

¹MRC Center for Immune Regulation, University of Birmingham

This video demonstrates the dissection and removal of the fetal thymus as well the preparation of ex vivo cultures of 2-dGuo-treated thymus.

Medicine

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Kenneth M. Tichauer¹, Robert W. Holt², Kimberley S. Samkoe³, Fadi El-Ghussein¹, Jason R. Gunn¹, Michael Jermyn¹, Hamid Dehghani⁴, Frederic Leblond¹, Brian W. Pogue^1,2

¹Thayer School of Engineering, Dartmouth College, ²Department of Physics and Astronomy, Dartmouth College, ³Darmouth Medical School, Dartmouth College, ⁴School of Computer Science, University of Birmingham

Diffuse fluorescence tomography offers a relatively low-cost and potentially high-throughout approach to preclinical in vivo tumor imaging. The methodology of optical data collection, calibration, and image reconstruction is presented for a computed tomography-guided non-contact time-domain system using fluorescent targeting of the tumor biomarker epidermal growth factor receptor in a mouse glioma model.

Neuroscience

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

Kentaro Kato¹, Alicia Hidalgo¹

¹Neurodevelopment Group, School of Biosciences, University of Birmingham

An injury paradigm using the Drosophila larval ventral nerve cord to investigate central nervous system regeneration and repair is described. Stabbing followed by laser scanning confocal microscopy in time-lapse and fixed specimens, combined with quantitative analysis with purposefully developed software and genetics, are used to investigate the molecular mechanisms of CNS regeneration and repair.