Supported Planar Bilayers for the Formation of Study of Immunological Synapses and KinapseSantosha Vardhana 1, Michael Dustin 1
1Helen and Martin S. Kimmel Center for Biology and Medicine at the Skirball Institute of Biomolecular, New York University - NYU
Supported planar bilayers are powerful tools that can be used to model the molecular interactions in an immunological synapse. Here, we show methods for anchoring cell adhesion proteins known to modulate synapse formation to the upper leaflet of the lipid bilyer and visualize synapse formation using TIRF microscopy.
